[Histonet] Tissue graft coming off of slide


Just wondering if anyone has any ideas to keep ePTFE (I will ask researcher what this stands for)
graft tissue from porcine from coming off of slides during antigen retrieval.  Actually, it is
starting to come off even after deparaffinization.  I told the researcher this is our last hope
that someone on the Histonet might have an idea.  Here is a summary of what I have tried.  I used
silanized slides (from Dako) with an overnight bake at 57C for tissue adherance plus an additional
10 minute 10% NBF soak to fix the tissue firmly on the slide after deparaffinization and before
antigen retrieval.  I have used low temperature antigen retrieval (LTAR) in the past for delicate
tissue, but if the tissue comes off after deparaffinization what is the point.  Is there something
else out there that I can try?  Thanks for any and all help.

Bob Meyer
Sr. Research Technologist
Northwestern University
Chicago, IL  60445


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