[Histonet] RE: Histonet Digest, Vol 29, Issue 15

From:"Ridhwaan Arries"

HENRY CHARLENE 

TRY NOVOCASTRA CD61. I HAVE JUST OPTIMISED IT FOR OUR LAB AND IT WORKS GREAT!!!



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, April 12, 2006 9:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 29, Issue 15


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Today's Topics:

   1. Dako unresponsive? (Sarah Holmes)
   2. histogel (Steven Coakley)
   3. Parathyroid hormone (Van Eyck, Deb)
   4. RE: histogel (Bartlett, Jeanine)
   5. Re: Dako unresponsive? (Chris Pomajzl)
   6. CD61 (Henry, Charlene)
   7. RE: CD61 (Drew Sally A.)
   8. Cell block prep (Linda Hines)
   9. Re: Dako unresponsive? (Dana Settembre)
  10. Sudan Red 7B staining procedure  (Kim Merriam)
  11. RE: Dako unresponsive? (Bell, Lynne)
  12. New edition of Polak and van Noorden	immunohistochemistry
      book (Gayle Callis)
  13. CSH meeting in May-Costa Mesa (Mitchell, Nancy K.)
  14. tendon processing/cutting (Mary Lou Norman)
  15. RE: CSH meeting in May-Costa Mesa (Morken, Tim - Labvision)
  16. RE: Sudan Red 7B staining procedure  (Monfils, Paul)
  17. re-freezing frozen sections (dgaupp@tulane.edu)
  18. RE: re-freezing frozen sections (Favara, Cynthia (NIH/NIAID) [E])
  19. Milestone microwave processor (Andrea C. Bilger)
  20. One question (Emre Kukurt)
  21. Auto dialers (remote alarms) for leica and tissue tek	VIP
      2000 (Vacca Jessica)
  22. RE: Auto dialers (remote alarms) for leica and	tissue tekVIP
      2000 (McGovern, Kevin)
  23. Re: Auto dialers (remote alarms) for leica and tissue	tek	VIP
      2000 (mari.ann.mailhiot@leica-microsystems.com)
  24. RE: QIHC (Patsy Ruegg)


----------------------------------------------------------------------

Message: 1
Date: Tue, 11 Apr 2006 12:25:49 -0500
From: "Sarah Holmes" 
Subject: [Histonet] Dako unresponsive?
To: 
Message-ID: <001501c65d8c$f9ee81c0$780a010a@wp.comcast.net>
Content-Type: text/plain;	charset="iso-8859-1"

Is anyone else having difficulty getting through to place orders with Dako?  

Thanks,
Sarah

------------------------------

Message: 2
Date: Tue, 11 Apr 2006 10:12:26 -0700 (PDT)
From: Steven Coakley 
Subject: [Histonet] histogel
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20060411171227.11273.qmail@web38206.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Ok, now I have successfull processed cells using histogel.  Do I dry and stain the slides as usual?
   
  Steve

		
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------------------------------

Message: 3
Date: Tue, 11 Apr 2006 12:21:23 -0500
From: "Van Eyck, Deb" 
Subject: [Histonet] Parathyroid hormone
To: "' histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"


I agree with Richard-----it would be nice to know when you order a product
that it is being discontinued.  I trialed several PTH antibodies and I ended
up liking the Novocastra PTH  NCL-PTH-488 - it is research use
only-------couldn't find a source for IVD PTH.   Hope this helps!
Deb Van Eyck
Cytology/Immunohistochemistry
Waukesha Memorial Hospital
725 American Avenue
Waukesha,WI 53188
phone:262-928-2112
fax:262-928-4849
deb.vaneyck@phci.org
 


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------------------------------

Message: 4
Date: Tue, 11 Apr 2006 13:22:10 -0400
From: "Bartlett, Jeanine" 
Subject: RE: [Histonet] histogel
To: "Steven Coakley" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Yep! 


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley
Sent: Tuesday, April 11, 2006 1:12 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] histogel

Ok, now I have successfull processed cells using histogel.  Do I dry and stain the slides as usual?
   
  Steve

		
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------------------------------

Message: 5
Date: Tue, 11 Apr 2006 12:36:02 -0500
From: "Chris Pomajzl" 
Subject: Re: [Histonet] Dako unresponsive?
To: "Sarah Holmes" ,	"HISTONET"
	
Message-ID: <003a01c65d8e$6a9e0d40$26fca8c0@CSP>
Content-Type: text/plain;	charset="iso-8859-1"

Dako is switching over to a new business system. So you are going to have
some trouble. Supposedly, they were going to be able to take calls as of
yesterday.

We placed an order at the end of March, and our supplies just arrived today.

Good luck.

----- Original Message ----- 
From: "Sarah Holmes" 
To: 
Sent: Tuesday, April 11, 2006 12:25 PM
Subject: [Histonet] Dako unresponsive?


Is anyone else having difficulty getting through to place orders with Dako?

Thanks,
Sarah
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 6
Date: Tue, 11 Apr 2006 12:38:57 -0500
From: "Henry, Charlene" 
Subject: [Histonet] CD61
To: 
Message-ID:
	<5CB39BCA5724F349BCB748675C6CA1A2099A1BEA@SJMEMXMB02.stjude.sjcrh.local>
	
Content-Type: text/plain;	charset="us-ascii"

Dako has discontinued the CD61 (Dako M0753) antibody that we have been
using for years and I was wondering if anyone knows of a good CD61 that
I could try as a replacement? I have tried the Ventana CD61 (Y2/51) and
Cell Marque CD61 (2f2) and they are not as good as our old antibody from
Dako.
Thanks,
 
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
 


------------------------------

Message: 7
Date: Tue, 11 Apr 2006 12:45:12 -0500
From: "Drew Sally A." 
Subject: RE: [Histonet] CD61
To: "Histonet" 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

We use the Cell Marque CD61 (clone 2f2) via Ventana, and it seems to
work well for us with mild CC1 on our VMS instruments.

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry,
Charlene
Sent: Tuesday, April 11, 2006 12:39 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] CD61


Dako has discontinued the CD61 (Dako M0753) antibody that we have been
using for years and I was wondering if anyone knows of a good CD61 that
I could try as a replacement? I have tried the Ventana CD61 (Y2/51) and
Cell Marque CD61 (2f2) and they are not as good as our old antibody from
Dako. Thanks,
 
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Tue, 11 Apr 2006 10:46:50 -0700 (PDT)
From: Linda Hines 
Subject: [Histonet] Cell block prep
To: histonet 
Message-ID: <20060411174650.78180.qmail@web33603.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi! All, 
  Thank-you for the responses regarding the thermometer's. Are any of you working with the Thin-prep and preparing cytology blocks upon completing the slide preparation? 
   My question is how we prepare the cell blocks. Any information is greatly appreciated. We are a new lab, and this is the first time I have'nt made my own smears etc.
   
  Thank-you
  Linda Hines HT (ASCP)

		
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------------------------------

Message: 9
Date: Tue, 11 Apr 2006 13:46:17 -0400
From: "Dana Settembre" 
Subject: Re: [Histonet] Dako unresponsive?
To: "Sarah Holmes" ,
	
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I was waiting too long on hold so I left a complete voicemail message as
they suggested and received a call back that they got my message.  The
processed my order.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> Sarah Holmes  04/11/06 1:25 PM >>>
Is anyone else having difficulty getting through to place orders with
Dako?  

Thanks,
Sarah
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Tue, 11 Apr 2006 10:53:08 -0700 (PDT)
From: Kim Merriam 
Subject: [Histonet] Sudan Red 7B staining procedure 
To: Histonet 
Message-ID: <20060411175308.21420.qmail@web50308.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello,
   
  I have found a reference for staining fat with sudan red 7B, it calls for a "saturated" solution of sudan red 7B in 70% ethanol.  Does anyone know what the saturation point of sudan red 7B is in 70% ethanol; a general guide would be very helpful!
   
  Also - has anyone used this procedure before and what were the results compared to a regular Oil Red O (both in isopropanol and/or propylene glycol).
   
  Thanks in advance,
  Kim


Kim Merriam, MA, HT(ASCP)
Novartis
Cambridge, MA
			
---------------------------------
Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates.

------------------------------

Message: 11
Date: Tue, 11 Apr 2006 14:01:36 -0400
From: "Bell, Lynne" 
Subject: RE: [Histonet] Dako unresponsive?
To: "Dana Settembre" ,	"Sarah Holmes"
	,	
Message-ID: 

We are also having a major problem getting through to Dako.   We were
told a couple of our items are on backorder for a few weeks.  I am
planning to call our account rep and voice my displeasure.  It would
have been nice to have received some communication regarding this
"changeover" and perhaps we could have upped our order before all hell
broke loose.
 
Lynne
 
Lynne A. Bell, HT (ASCP)
Laboratory
Central Vermont Hospital
P.O. Box 547
Barre, VT 05641
(802)371-4122

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dana
Settembre
Sent: Tue 4/11/2006 1:46 PM
To: Sarah Holmes; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Dako unresponsive?



I was waiting too long on hold so I left a complete voicemail message as
they suggested and received a call back that they got my message.  The
processed my order.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> Sarah Holmes  04/11/06 1:25 PM >>>
Is anyone else having difficulty getting through to place orders with
Dako? 

Thanks,
Sarah
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 12
Date: Tue, 11 Apr 2006 12:06:04 -0600
From: Gayle Callis 
Subject: [Histonet] New edition of Polak and van Noorden
	immunohistochemistry book
To: Histonet@lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20060411115552.01b3bc98@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

If you are looking for a concise, introductory book to immunohistochemical 
staining, I strongly suggest the long awaited new edition of:

Introduction to ImmunoCytochemistry, 3rd Edition, JM Polak and S van 
Noorden, ISBN 185996 208 4.  A paperback with 179 pages.

This new edition was easy to follow and had excellent basic 
information.  The appendix was packed with Technical notes on buffers, 
blocks, chromogens, etc.

It cost ~$61 from Amazon.com
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)





------------------------------

Message: 13
Date: Tue, 11 Apr 2006 11:09:00 -0700
From: "Mitchell, Nancy K." 
Subject: [Histonet] CSH meeting in May-Costa Mesa
To: 
Message-ID:
	<8EB1459EA20E4E498AC2E05378F8F30A536EDA@mind.mc.ad.lluahsc.org>
Content-Type: text/plain;	charset="iso-8859-1"

If any of you California Histoechs are out there, I am having problems getting a copy of the workshops that are going to be available.
Who should I contact?

Nancy Mitchell, HT ASCP
Histology Supervisor
Loma Linda Pathology Medical Group
11370 Anderson Street Ste 2960
Loma Linda, CA 92354
909-558-2012  FAX-909-796-3667




------------------------------

Message: 14
Date: Tue, 11 Apr 2006 14:21:15 -0400
From: Mary Lou Norman 
Subject: [Histonet] tendon processing/cutting
To: Histonet@lists.utsouthwestern.edu, Stacy.Semevolos@oregonstate.edu
Message-ID:
	<5.2.1.1.2.20060411140143.02cbf850@postoffice9.mail.cornell.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed


Thanks Elizabeth and Gayle for the tendon info. I've done the longer 
soaking which does help quite a bit. I've got big hunky horse pieces. My 
question today: After the slide has dried all night on the warming tray I 
still see white tissue, definitely not as opaque as I'd like. Any 
suggestions on making sure the tissue doesn't fall off? I'm just the cutter 
so I don't know what IHC is going to be done. Could I put them in the oven 
60o? What's wrong with melting the paraffin?

Stacy, can I put them in the oven?

Thanks,
Mary Lou Norman





------------------------------

Message: 15
Date: Tue, 11 Apr 2006 14:24:58 -0400
From: "Morken, Tim - Labvision" 
Subject: RE: [Histonet] CSH meeting in May-Costa Mesa
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<0556BE8AC5551E4E8AF6BB9E42509BA20328D843@usca0082k08.labvision.apogent.com>
	
Content-Type: text/plain

Nancy, you can download the program and registration information from
www.californiahistology.org

Tim Morken




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell,
Nancy K.
Sent: Tuesday, April 11, 2006 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CSH meeting in May-Costa Mesa


If any of you California Histoechs are out there, I am having problems
getting a copy of the workshops that are going to be available. Who should I
contact?

Nancy Mitchell, HT ASCP
Histology Supervisor
Loma Linda Pathology Medical Group
11370 Anderson Street Ste 2960
Loma Linda, CA 92354
909-558-2012  FAX-909-796-3667


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 16
Date: Tue, 11 Apr 2006 14:59:07 -0400
From: "Monfils, Paul" 
Subject: RE: [Histonet] Sudan Red 7B staining procedure 
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<09C945920A6B654199F7A58A1D7D1FDE017176CD@lsexch.lsmaster.lifespan.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

I don't have an exact figure, but according to one of my older texts
(Histopathologic Technic and Practical Histochemistry by Lillie and
Fullmer), 4.25 grams Sudan Red 7B will dissolve in 100 ml absolute ethanol.
So the solubility in 70% ethanol should be substantially less than that.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Kim
> Merriam
> Sent: 	Tuesday, April 11, 2006 10:53 AM
> To: 	Histonet
> Subject: 	[Histonet] Sudan Red 7B staining procedure 
> 
> Hello,
>    
>   I have found a reference for staining fat with sudan red 7B, it calls
> for a "saturated" solution of sudan red 7B in 70% ethanol.  Does anyone
> know what the saturation point of sudan red 7B is in 70% ethanol; a
> general guide would be very helpful!
>    
>   Also - has anyone used this procedure before and what were the results
> compared to a regular Oil Red O (both in isopropanol and/or propylene
> glycol).
>    
>   Thanks in advance,
>   Kim
> 
> 
> Kim Merriam, MA, HT(ASCP)
> Novartis
> Cambridge, MA
> 			
> ---------------------------------
> Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



------------------------------

Message: 17
Date: Tue, 11 Apr 2006 14:22:03 -0500
From: dgaupp@tulane.edu
Subject: [Histonet] re-freezing frozen sections
To: histonet@lists.utsouthwestern.edu
Message-ID: <1144783323.443c01db190b1@webmail.tulane.edu>
Content-Type: text/plain; charset=ISO-8859-1

Histonet:

I have tissues(monkey brain) that were fixed(4%Paraformaldehyde),
cryprotected(20% sucrose), and flash froze(-70C isopentane).  Unfortunately,
mothernature(hurricane katrina) came threw and destroyed everything.  This
tissues sat for 3-4 months at blistering temps in the same position(melted OCT)
& some even had a surprise for me(fungus).  When the University let us come back
into our building, I rinsed off OCT and put the tissues in 10%NBF at room temp. 
The tissue was immersed for 3-4months in fixative.  I rinsed the tissue and once
again, flash froze one of the sections, and they cut horribly.  I don't know
what to do.  I am now tempting to paraffin process. I prefer to do frozens
because its easier to get almost every section of tissue(one sections I usually
get 500-700 slides).  As most of you know, brain likes to explode in water, so I
can do a better job if they are frozen.

Any help, I would appreciate!

Dina


Dina D. Gaupp, B.S., M.T.
Medical Research Specialist
Center for Gene Therapy
Tulane University Health Sciences Center
JBJ Bldg/Rm 658, SL-99
1430 Tulane Avenue
New Orleans, LA 70112
Lab: 504.988.1194
Fax: 504.988.7710
Email: dgaupp@tulane.edu



------------------------------

Message: 18
Date: Tue, 11 Apr 2006 17:43:52 -0400
From: "Favara, Cynthia \(NIH/NIAID\) [E]" 
Subject: RE: [Histonet] re-freezing frozen sections
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I have done some squirrel monkey brain in paraffin with good results -
happy to help with processing schedules etc if you like. Let me know

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

Disclaimer: 
The information in this e-mail and any of its attachments is
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-----Original Message-----
From: dgaupp@tulane.edu [mailto:dgaupp@tulane.edu] 
Sent: Tuesday, April 11, 2006 12:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re-freezing frozen sections

Histonet:

I have tissues(monkey brain) that were fixed(4%Paraformaldehyde),
cryprotected(20% sucrose), and flash froze(-70C isopentane).
Unfortunately,
mothernature(hurricane katrina) came threw and destroyed everything.
This
tissues sat for 3-4 months at blistering temps in the same
position(melted OCT)
& some even had a surprise for me(fungus).  When the University let us
come back
into our building, I rinsed off OCT and put the tissues in 10%NBF at
room temp. 
The tissue was immersed for 3-4months in fixative.  I rinsed the tissue
and once
again, flash froze one of the sections, and they cut horribly.  I don't
know
what to do.  I am now tempting to paraffin process. I prefer to do
frozens
because its easier to get almost every section of tissue(one sections I
usually
get 500-700 slides).  As most of you know, brain likes to explode in
water, so I
can do a better job if they are frozen.

Any help, I would appreciate!

Dina


Dina D. Gaupp, B.S., M.T.
Medical Research Specialist
Center for Gene Therapy
Tulane University Health Sciences Center
JBJ Bldg/Rm 658, SL-99
1430 Tulane Avenue
New Orleans, LA 70112
Lab: 504.988.1194
Fax: 504.988.7710
Email: dgaupp@tulane.edu

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 19
Date: Tue, 11 Apr 2006 20:04:54 -0400
From: "Andrea C. Bilger" 
Subject: [Histonet] Milestone microwave processor
To: 
Message-ID: <058f01c65dc4$baebb320$0300a8c0@andrea>
Content-Type: text/plain;	charset="iso-8859-1"

We had the RHS I in our lab for a couple of days to test it out.  It performed well.  The PA's didn't change how they grossed the tissue.  This was a big plus.  No need for special tools and tiny sized tissues. You can program it to the largest size specimen being processed.   Our lab is looking into getting at least one of the small units and we may go for the new Pathos they just came out with.  The Pathos is larger and automatically changes the solutions.  No need for manually changing them like you do with the RHS I.  Now if we can just convince the top brass to give us the money for one of them.

Andrea Bilger 


------------------------------

Message: 20
Date: Wed, 12 Apr 2006 07:40:41 -0700 (PDT)
From: Emre Kukurt 
Subject: [Histonet] One question
To: histonet@lists.utsouthwestern.edu
Message-ID: <20060412144041.65778.qmail@web31001.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello Histonetters,
Im new here, not sure whether or no my problem
appropriate for the list topic.

I have treated low electric current on grafted plants.
Then, they were sectioned, prepared and photographed
either light or electron microscope.

I need to show them to an expert on plant histology
who can interpret the pictures in order to decide what
(sub)celullar changes appeared before/after electric
current applied.

Can some please one help me ? 

Best regards 
- Emre





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------------------------------

Message: 21
Date: Wed, 12 Apr 2006 11:08:51 -0400
From: "Vacca Jessica" 
Subject: [Histonet] Auto dialers (remote alarms) for leica and tissue
	tek	VIP 2000
To: 
Message-ID:
	<41E16A15CE78374EA45B57E0F94339B84B5ADC@ORLEV01.hca.corpad.net>
Content-Type: text/plain;	charset="iso-8859-1"


Another day has gone by with the machine alarming over the weekend and nobody knew until it was too late. Does anyone know about the remote alarms for both of these types of processors? Do I need 2 separate ones or can biomed hook up 1 remote to 2 machines? Where can I find this product? Save a histotech from another Manic Monday! Thanks
Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr
Brandon FL 33511
(813) 681-5551 ext 2454
(813) 571-5193
(813) 681-5169 Fax



------------------------------

Message: 22
Date: Wed, 12 Apr 2006 10:26:06 -0500
From: "McGovern, Kevin" 
Subject: RE: [Histonet] Auto dialers (remote alarms) for leica and
	tissue tekVIP 2000
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=iso-8859-1

Jessica,

You may need two boxes, depending on how the instruments signal they are in an alarm state. In many cases, they simply short together their output wires, in some, they send out 5VDC. We have a VIP E-150 with a home-brewed alarm box that works well. Your local biomed should be able to cook one up if he has the electrical diagrams for the units. It ain't rocket science!  Good luck.

Kevin

Kevin S. McGovern, BMETII
Good Samaritan Hospital
Clinical Engineering Dept.
10 East 31st Street
Kearney, NE 68836
Phone: (308) 865-7051
Fax:      (308) 865-2914
e-Mail: kevinmcgovern@catholichealth.net 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vacca
Jessica
Sent: Wednesday, April 12, 2006 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Auto dialers (remote alarms) for leica and tissue
tekVIP 2000



Another day has gone by with the machine alarming over the weekend and nobody knew until it was too late. Does anyone know about the remote alarms for both of these types of processors? Do I need 2 separate ones or can biomed hook up 1 remote to 2 machines? Where can I find this product? Save a histotech from another Manic Monday! Thanks
Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr
Brandon FL 33511
(813) 681-5551 ext 2454
(813) 571-5193
(813) 681-5169 Fax

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------------------------------

Message: 23
Date: Wed, 12 Apr 2006 11:04:46 -0500
From: mari.ann.mailhiot@leica-microsystems.com
Subject: Re: [Histonet] Auto dialers (remote alarms) for leica and
	tissue	tek	VIP 2000
To: "Vacca Jessica" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=US-ASCII

Jessica

I have asked Mike Gallo to give you a call in regards to your alarm for the
Leica processor. However, your biomedical engineers should be able to wire
the alarm  for you.

If you have questions just give me a call.

Best Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com


                                                                           
             "Vacca Jessica"                                               
                                                        To 
             Sent by:                   
             histonet-bounces@                                          cc 
             lists.utsouthwest                                             
             ern.edu                                               Subject 
                                       [Histonet] Auto dialers (remote     
                                       alarms) for leica and tissue tek    
             04/12/2006 10:08          VIP 2000                            
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           





Another day has gone by with the machine alarming over the weekend and
nobody knew until it was too late. Does anyone know about the remote alarms
for both of these types of processors? Do I need 2 separate ones or can
biomed hook up 1 remote to 2 machines? Where can I find this product? Save
a histotech from another Manic Monday! Thanks
Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr
Brandon FL 33511
(813) 681-5551 ext 2454
(813) 571-5193
(813) 681-5169 Fax

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 24
Date: Wed, 12 Apr 2006 10:43:25 -0600
From: "Patsy Ruegg" 
Subject: RE: [Histonet] QIHC
To: "'Marian powers'" ,
	
Message-ID: <200604121643.k3CGhNka013874@chip.viawest.net>
Content-Type: text/plain;	charset="us-ascii"

Marian you  can join the NSH IHC Resource Group online at www.ihcrg.org 
We have a list of references and you can actually access the ws on taking
the QIHC exam presented by myself and Ethel Macrea at the NSH S/C last year.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marian
powers
Sent: Monday, April 10, 2006 7:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QIHC

Looking for a good IHC reference to study for the QIHC?

Thanks in advance,

Marian
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------------------------------

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End of Histonet Digest, Vol 29, Issue 15
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