[Histonet] Immunofluorescence - CAP 21850

From:"Chris Pomajzl"

Revisiting this CAP question regarding appropriate controls for IF:

I understand this question to read that internal controls can be used on renal bx's, but what about skin bx's? In speaking with one of our dermatopathologists, he has asked some colleagues, and they claim that a positive tissue control needs to be run for each antibody tested. Is this correct? And if so, how does one acquire positive tissue other than from a positive case? If we do not have any positive controls at the moment, how can we do IF's and be of positive/negative fluorescence?

I am concerned because we have not been running positive controls for several years now. I know that this is a Phase II NEW question from CAP, so I want to make sure that we address the issue properly.

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