[Histonet] Humidity chamber
Thermo Electron makes a great system with a humidity chamber and a
coverplate that I have used for many years. The slides are held with
the plastic coverplate(the coverplates are suppose to be disposable, but
I rinse them with only DI water and reuse them many times) I use 100ul
of antibody (primary, secondary- etc, normally, but have managed to use
as little as 90ul) to cover almost the whole slide. For buffer wash I
fill up the top of the chamber with a squeeze bottle of PBS and allow to
run through in about 5 minutes. I load the slides add primary, wash,
secondary etc and only take them out for chromagen staining.
The racks worked great when I had the positive control at the top of the
slide and the test below. I do fluorescence secondaries in the
coverplates, but I not do DAB in the coverplates, but flat on the
For HRP frozens I use the Dako endogenous peroxidase block in the
Sequenza racks. I also use the Dako avidin biotin block in the
coverplates when necessary.
I usually incubate primary overnight in the fridge and the chambers stay
humid for at least 48 hours. It takes a bit of practice to load the
plates, but it is so worth it.
Sequenza Racks - # 73310017 (Holds 10 coverplate assemblies)
Coverplates - 25/pk = # 72110017
50/pk = # 7219950
250/case = # 72110013
Donna Harclerode, HT, (ASCP), HTL, QIHC
Scientist / Immunohistochemistry
3020 Callan Rd.
San Diego, CA 92121
858-458-0900 ext 5416
Histonet mailing list
<< Previous Message | Next Message >>