[Histonet] Hematoxylin staining of frozen samples, where are the nuclei?

From:Guillermo Palao

Dear histonetters,
  When I stain samples placed on VWR frosted slides with Gills hematoxylin, nuclei are nicely stained. However, when the same samples are counterstained after passing through all necessary steps involved in immunohistochemistry, hematoxylin staining of nuclei is simply horrible. Is it possible that nuclei are lost in the different washing steps? Please put forward any suggestions you might have.


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