Re: [Histonet] Taft's Method For Nucleic Acids


What are you staining for?

Just curious. MGP used to be used to demonstrate plasma cells (RNA in the
cytoplasm), but IHC is SOOO much better.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak MI 48073
----- Original Message -----
From: "Farley, Sunni R" 
Sent: Sunday, April 10, 2005 4:22 PM
Subject: [Histonet] Taft's Method For Nucleic Acids

> I am looking for information regarding Methyl Green-Pyronin staining for
> nucleic acids.  I have contacted Rowley Biochemical Inc (Danvers, MA)
> their Methyl Green Pyronin stain and Differentiating Solution.  They
> provided me with the chemical composition of these solutions and also the
> protocol
> for Taft's Method  for Nucleic Acids.  Does anybody routinely use these
> items and this method?  Also, have you used them on B5-fixed tissue?
> Any information and/or suggestions would be much appreciated.  Thank you
> your time!
> Sunni R. Farley
> Histotechnician
> Pathology Department
> Seattle Cancer Care Alliance
> 825 Eastlake Ave East
> Seattle, Washington 98109
> (206) 288-1312
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