1. Is your peroxide fresh? It turns to water over time.
2. Call Pierce and see if they have any ideas.

Geoff

Till, Renee wrote:

>Is there some secret to this I haven't figured out? I ordered the Pierce
>Metal Enhanced DAB, thinking I might like the brownish/black better, and
>it's supposedly more sensitive. I've now tried it twice and ended up
>ruining two whole days work because it did absolutely nothing. No brown,
>no black. I've read and reread the mixing instructions and I can't see
>where I could've went wrong. I dug some regular DAB out of a kit and it
>won't even work after the other has been on the slides. Any ideas? These
>are identical immuno runs that worked perfectly before I switched DAB.
>
> 
>
>Renee' Till, HT
>
> 
>
>Arkansas Childrens Nutrition Center
>
>1120 Marshall
>
>Little Rock, AR 
>
>72202
>
> 
>
>(501) 364-2774
>
> 
>
>
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-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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