Re: [Histonet] Mallory PTAH and Zenkers
No, you would not have to do the iodine and sodium thiosulfate step if you
fix/post-mordant in something other than mercury.
If you want, fix the tissue in formalin, process/embed/section as usual, and
then postmordant in Bouin at 60 degree C for 1 hour before staining. Same as
doing a trichrome. Wash in running water afterwards until the yellow color
is gone. Stain with PTAH. Works great.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Perry, Margaret"
To:
Sent: Tuesday, April 12, 2005 6:04 PM
Subject: [Histonet] Mallory PTAH and Zenkers
Hello, I have a question about the Mallory's PTAH. If I use the zenkers
without mercuric chloride do I still need to use Gram's iodine and
sodium thiosulfate? I assume not but need to be sure.
Thanks for the help.
Margaret Perry HT
South Dakota University
Veterinary Science
Histopathology
Brookings SD
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