I use a H&E counterstain for Von Kossa.  I use gills 3 hematoxylin and do
not destain in acid alcohol/ I do blue briefly in ammonia water, wash well
and then use a 0.2% aqueous eosin for 30 min., dehydrate quickly thru 95%
alcohols, 100 and xylene then permount.  This method will stain osteoid on
bone viewed by fluorescence UV.  It is very nice for histomorphometry by
image analysis as only the osteoid lights up and the rest of the tissue
(calcified bone and blue nuclei) is dark under UV, makes it really easy to
measure osteoid seams.
Patsy 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich,
Ruth A (NIH/NIDCR)
Sent: Monday, April 18, 2005 12:28 PM
To: Gary Radice; 'Jennifer MacDonald'
Cc: Histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] neutral stains for protozoa

You can stain with the Von Kossa with a nuclear fast red counterstain.
Ruth Yaskovich
N.I.H.


> ----------
> From: 	Jennifer MacDonald
> Sent: 	Monday, April 18, 2005 2:18 PM
> To: 	Gary Radice
> Cc: 	Histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] neutral stains for protozoa
> 
> There is an iron hematoxylin procedure for protozoa.  It is in the 
> AFIP manual.  I don't have it.  It is Heidens or something similar.
> 
> 
> 
> 
> 
> 
> Gary Radice 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 03/31/2005 06:47 AM
> 
> To
> Histonet@lists.utsouthwestern.edu
> cc
> 
> Subject
> [Histonet] neutral stains for protozoa
> 
> 
> 
> 
> 
> 
> I have a colleague who is studying a calcified protozoan. She is 
> specifically interested in calcification, and she is looking for 
> stains she can use that are not acidic and that don't require acid 
> differentiation steps, so that she doesn't inadvertently decalcify the 
> specimens.
> 
> Any thoughts about nuclear or counterstains that might work in this 
> situation?
> 
> 
> 
> Gary P. Radice gradice@richmond.edu
> Department of Biology            804-289-8107 (voice)
> University of Richmond           804-289-8233 (FAX)
> Richmond VA 23173                http://www.richmond.edu/~gradice
> 
> 
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> 

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