[Histonet] Immunofluorescence on salt-split skin

From:"Cory Collins"

I found out today that my lab will be doing IF's on salt-split skin biopsies
in the near future for bullous pemphigoid.  Can anyone that is currently
doing this give me some insight into the sodium chloride concentration, the
temperature,  and the amount of time you leave the skin in the solution?
Also, does the skin sperate on its own or do you have to use forceps to
seperate the dermis from the epidermis?



Cory Collins, HT (ASCP) QIHC
Immunohistochemistry Supervisor
8267 Elmbrook Drive Suite 100
Dallas, TX 75247
214-638-2000 ext 2027
214-237-1730 Fax
=0ATo learn more about ProPath, please visit http://www.ProPathLab.com 


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