Re: [Histonet] unsuccessful PNA staining

From:"Jan Shivers"

There may also be a difference in the type and/or amounts of lectins
expressed by species other than humans (I assume the book was about human
lectin staining).  I found this to be true when trying to stain non-primate
mammalian endothelium with Ulex europaeus.

The inquirer should first be sure that the glycoprotein he's trying to label
on the bovine spleen/lymph node cells with PNA lectin is known to be present

----- Original Message ----- 
From: "Gayle Callis" 
To: "Subhash Verma" ; 
Sent: Monday, April 04, 2005 11:09 AM
Subject: [Histonet] unsuccessful PNA staining

> The method I sent is the one that comes from the Lectin Histochemistry
> (Brook, Leathem and Schumacher ISBN# 1 85996 100 2)  book and also from a
> workshop by Barb Wright (a magician at immuno and lectin staining).   Her
> method was taken from this book.    What I sent to you are guidelines, and
> you may need to work with concentrations (lectin-HRP), incubations, etc to
> make it work.
> If you tell us exactly how you did the staining, then more suggestions can
> be made especially from others who do FFPE lectin work.  My successful
> lectin staining has been done on frozen sections and NOT FFPE and I used
> Vectors lectin.
> The book, by the way, is inexpensive and wonderful source of information.
> At 03:44 AM 4/4/2005, you wrote:
> >Hi Gayle,
> >
> >I have been unsuccessful to stain germinal centres from bovine spleen and
> >lymphnodes (formalin fixed parafin embedded sections)with lectin
> >conjuagated to HRP as per the protocol suggetsed by you. Any suggestions,
> >please.
> >Thanks.
> >SV
> >--
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> _______________________________________________
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