Again, the issue is not with tissue adhesion, it is the uniformity of
the coating. The XT uses air to mix the reagent while its on the slides
and if the coating isn't uniform there is spotty staining.

>>> "Elizabeth Chlipala"  04/07/05 2:09 PM >>>
For all this is worth, here is my experience with plus slides.  I have
found no differences in tissue attachment in the following brands of
plus slides -
 
VWR, Fisher, Stat Labs superfrost plus and histobond slides - I would
use any one of these for immuno's without fear of losing my sections. 

 
I have tried Surgipaths snowcoat x-tra and some plus slide that
Mercedes
Medical sells (made in China).  In my experience the immuno's I did
with
the slides from surgipath and Mercedes medical, the tissue sections
fell
of the slides (with both automated and very careful handling of manual
methods)  I would never use those slides for immunos or even fixed
frozen sections.  I cut some fixed tissue for frozen and used
surgipaths
snowcoat extra and the sections fell off, but adhered just fine to the
the StatLab histobond slides.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com 
www.premierlab.com 
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet