Re: [Histonet] Universal (protein) block for IHC
I noticed a decrease in specific staining when I plugged it into my
protocol. I made it myself and it was a big pain. Buy it! Try increasing
antibody concentration on a few slides and see what happens. I never use
a block with my secondary.
Johnson, Teri wrote:
>Has anybody converted to using a universal protein block (casein) for
>their immunohistochemistry protocols rather than using species specific
>normal sera? For those who have made the conversion, did you have to
>make any changes to your methodology or did you just plug it in to your
>protocol and run? Did you notice any difference in the staining
>intensity for known antibodies (stronger, weaker, no change)? Are you
>also using it as a secondary antibody diluent or for washes? Finally,
>are you using commercially available reagent or are you making your own?
>Thanks so much!
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri 64110
>Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>