Re: [Histonet] RCC Antibody Help

From:"Richard Cartun"

We used to use Novocastra's RCC mAb, but were unhappy with its
performance.  Try Lab Vision's ( mAb; I think you will
be satisfied.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Sharon Chase"  04/15/05 08:01AM >>>
 I am trying to establish a protocol for Vector's renal cell carcinoma
antibody. The data sheet states trypsin digestion for 30 minutes. I
obtain negative results with trypsin digestion using normal kidney and
clear cell carcinoma as controls. 
I then did HIER retrieval using Biocare's reveal and borg solutions in
their decloaker. The normal kidney's proximal tubules stained but the
clear cell was patchy. Repeating the run on the following day, using
same reagents and working dilution, I obtained weak results with
kidney and negative tumor cells. 
A fresh daily working dilution(1/50), I can obtain constant normal
kidney staining but usually negative tumor cells.
I tried another tumor control block and leaving the antibody
 Normal kidney is fine.
Open to any suggestions.
Sharon Chase
Atlantic Health Science Center
Saint John NB
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