Re: [Histonet] Mouse femurs and tibias fixation time
I let them fix for IHC 24-48 hours. Are you going to decalcify? I
decalcify in a formic acid decal, then trim away some of the cortical bone
for the perspective I want (I'm doing whole legs). Since they bow to the
right or left, I can get the surface I want. Trimming away the cortical
bone enables the processing solutions to penetrate much better, and I have
no artifacts.
Jacqueline M. O'Connor HT(ASCP)
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor@abbott.com
"Grant, Debra"
Sent by: histonet-bounces@lists.utsouthwestern.edu
04/08/2005 02:17 PM
To: "Histonet"
cc:
Subject: [Histonet] Mouse femurs and tibias fixation time
Hi all,
I am processing some mouse femurs and tibias for IHC. Any
recommendations as to amount of fixation time for Zinc formalin before
paraffin processing?
Thanks in advance!
Cheers!
Debby Grant
Histology Specialist I
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
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