Re: [Histonet] Mouse femurs and tibias fixation time

From:"Jackie M O'Connor"

I let them fix for IHC 24-48 hours.  Are you going to decalcify?  I 
decalcify in a formic acid decal, then trim away some of the cortical bone 
for the perspective I want (I'm doing whole legs). Since they bow to the 
right or left, I can get the surface I want.  Trimming away the cortical 
bone enables the processing solutions to penetrate much better, and I have 
no artifacts.

Jacqueline M. O'Connor HT(ASCP)
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL

"Grant, Debra" 
Sent by:
04/08/2005 02:17 PM

        To:     "Histonet" 
        Subject:        [Histonet] Mouse femurs and tibias fixation time

Hi all,

I am processing some mouse femurs and tibias for IHC. Any
recommendations as to amount of fixation time for Zinc formalin before
paraffin processing?

Thanks in advance!


Debby Grant
Histology Specialist I 
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>