Re: [Histonet] Is TUNEL as waste of time?
What about apoptotic CK-18 antibody? It is supposed to be more sensitive
than TUNEL. You can use it with Ki-67 antobody.
Good luck,
Kelly
Satoshi Akima wrote:
> Dear all,
>
> I am looking into the Roche TUNEL kit and have been getting various
> opinions. Some say it is easy and reviewers will ask for a TUNEL stain
> in preference to other methods such as staining for caspase-3. However
> many people find that they get non-specific staining and even where
> things seem to have gone well you get a great many cells that seem
> absolutely healthy staining up positive.
>
> Do people think that TUNEL is a sham technique that ivory tower
> reviewers who never get their hands dirty in the lab ask for, never
> realising how hopeless the technique is? Or is it still a genuinely
> useful tool?
>
> Toshi Akima
> PhD Student
> Centre for Transplantation and Renal Research
> Westmead Millenium Institute
> Sydney, Australia
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>