Re: [Histonet] Is TUNEL as waste of time?

From:Kelly D Mcqueeney

What about apoptotic CK-18 antibody? It is supposed to be more sensitive 
than TUNEL. You can use it with Ki-67 antobody.
Good luck,

Satoshi Akima wrote:

> Dear all,
> I am looking into the Roche TUNEL kit and have been getting various 
> opinions. Some say it is easy and reviewers will ask for a TUNEL stain 
> in preference to other methods such as staining for caspase-3. However 
> many people find that they get non-specific staining and even where 
> things seem to have gone well you get a great many cells that seem 
> absolutely healthy staining up positive.
> Do people think that TUNEL is a sham technique that ivory tower 
> reviewers who never get their hands dirty in the lab ask for, never 
> realising how hopeless the technique is? Or is it still a genuinely 
> useful tool?
> Toshi Akima
> PhD Student
> Centre for Transplantation and Renal Research
> Westmead Millenium Institute
> Sydney, Australia
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