Trisha,

We have successfully used Tris-EDTA (ph 9.2) as a HIER
method with certain antibodies. Which  in particular
are you testing ?

As far as the water goes, I always believe that
Distilled, or even double distilled if you have it -
is best for all solution preparation. Water from the
faucet can introduce salts and minerals that may
affect your solution's abilities.

KP

--- Trisha Emry  wrote:
> Is it necessary to use distilled H20 for EDTA or can
> you use tap?
> 
> Is anyone using heat with EDTA?  Please send
> details.
> 
> Thank you,
> 
> Trisha Emry
> U of WA, Seattle
> 
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