RE: [Histonet] need a good protocol for apoptosis

From:"Elizabeth Chlipala"

You could look at performing tunel in which you would need to purchase a
kit or you could stain immunohistochemically for cleaved caspase 3,
which is a marker of apoptosis.  The heat should not affect the
detection of apoptosis in fixed tissue.  To induce apoptosis in cell
culture you can place the cells at 36C for 30 minutes, but you need to
control the temp.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sayeed,
Mohammed
Sent: Tuesday, April 05, 2005 10:56 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] need a good protocol for apoptosis
 
I need a protocol for apoptosis for paraffin embedded tissue. Has
incubating
paraffin sections at 50 degrees for a certain length of time   any
effect on
the out come of the apoptosis staining? Thanks.
 
M. Sayeed
Histology lab Manager
Dept. Of Pathology
Prostate research (Spore)
Baylor College Of Medicine
Phone: 713-798-3650
 
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