RE: [Histonet] microns on frozen sections of lung

From:"Alan Bright"

Teresa,

	For frozen sections of lung -18 to -20 C, specimen temperature.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Flores, Teresa [mailto:tflore@lsuhsc.edu] 
Sent: 04 April 2005 14:29
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microns on frozen sections of lung


Is there an "ideal temperature" to frozen section lung tissue? Any input would surely be appreciated, as I usually frozen section, at 4-6 microns, renal, skin and heart between -15 and -12 o C degrees, with not a lot of problem. But this last time, lung tissue was included and I had such great difficulty in obtaining a decent frozen section and was almost embarrassed to turn in what I was able to obtain. Teresa Flores LSUHSC EM Lab New Orleans, LA _______________________________________________
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