RE: [Histonet] bacteria stain in plant tissue

From:"Favara, Cynthia (NIH/NIAID)"

I have no experience with plant tissue so this is just a thought!

Have you tried just a plan gram Stain?

Others would be Brown-Hopps and Gram Twort. I like both of these in tissue.

Good Luck!
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Walters, Katherine S [mailto:katherine-walters@uiowa.edu] 
Sent: Tuesday, April 05, 2005 12:18 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] bacteria stain in plant tissue 

Hi,

Has anyone had any experience staining for bacteria in plant tissues?
In Ruzin's book basic fuchsin is used, but it also stains nuclei, mucin
and lignified tissues.  My bacteria of interest is gram negative.

Thanks for any ideas you can give me!!

Kathy


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