RE: [Histonet] Universal (protein) block for IHC

From:"Elizabeth Chlipala"


I have been using dako's serum free protein block for about 1.5 years
now.  When I switched I just replaced that reagent with the blocking
agent I was currently using.  I have not noticed any decrease in
staining intensity.  I did see decreases in background staining, which
could be due to the serum free protein block or improvement in my own
technique, since I'm always learning about better ways to do things.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Johnson,
Sent: Wednesday, April 06, 2005 2:34 PM
To: Histonet
Subject: [Histonet] Universal (protein) block for IHC

Has anybody converted to using a universal protein block (casein) for
their immunohistochemistry protocols rather than using species specific
normal sera?  For those who have made the conversion, did you have to
make any changes to your methodology or did you just plug it in to your
protocol and run?  Did you notice any difference in the staining
intensity for known antibodies (stronger, weaker, no change)?  Are you
also using it as a secondary antibody diluent or for washes?  Finally,
are you using commercially available reagent or are you making your own?
Thanks so much!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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