RE: [Histonet] Mouse femurs and tibias fixation time

From:"Elizabeth Chlipala"


I agree with Jackie we fix for 24-48 hours and then decal in 5% formic
acid.  We section the knees in a frontal plane, so we remove most of the
muscle prior to placement in decal.  


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Grant,
Sent: Friday, April 08, 2005 12:18 PM
To: Histonet
Subject: [Histonet] Mouse femurs and tibias fixation time

Hi all,

I am processing some mouse femurs and tibias for IHC. Any
recommendations as to amount of fixation time for Zinc formalin before
paraffin processing?

Thanks in advance!


Debby Grant
Histology Specialist I  
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110

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