We use have used Protease digestion with success, though we do not use the Antibody much.  Protease is Sigma P-8038,  Type XXIV.  10mg in 10ml of PBS    20 mins

Dave
Histology
Christie 
Manchester UK


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Coolen,
Neeltje
Sent: 07 April 2005 09:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Laminin antibody


Dear Histonetters,
 
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
another antibody? 
 
Thank you for your help.
 
Neeltje Coolen, PhD student
VSBN
Zeestraat 26
1941 AJ Beverwijk
The Netherlands
 
 
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