RE: [Histonet] Laminin antibody

From:"Edmondson David (RBV) NHS Christie Tr"

We use have used Protease digestion with success, though we do not use the Antibody much.  Protease is Sigma P-8038,  Type XXIV.  10mg in 10ml of PBS    20 mins

Manchester UK

-----Original Message-----
[]On Behalf Of Coolen,
Sent: 07 April 2005 09:37
Subject: [Histonet] Laminin antibody

Dear Histonetters,
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
another antibody? 
Thank you for your help.
Neeltje Coolen, PhD student
Zeestraat 26
1941 AJ Beverwijk
The Netherlands
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