RE: [Histonet] Does anyone charge for running immuno controls?

From:"Horn, Hazel V"

We don't charge for controls.   I would consider them part of the test.
I've never heard of charging for them

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital - Changing Children's Lives

Phone - 501.364.4240
Fax - 501.364.3912 

Visit us on the web at      www.

-----Original Message-----
[] On Behalf Of Flores,
Sent: Thursday, April 07, 2005 9:32 AM
Subject: [Histonet] Does anyone charge for running immuno controls?

Our EM Lab has never charged for controls on performing
immunofluorescent studies. 
We do not use a negative and a positive control for each antigen (but I
do understand that there is a new CAP regulation addressing this) but
rather, our EM lab saves previously reported positive strongly positive
for IgG, IgM, IgA, and C3.
We section through leftover tissue (either renal or skin) and fix in hi
grade acetone and save slides in a -70oc freezer. 
When sectioning a case for immunofluorescent studies, we pull two slides
out. On one of the slides with control section we  place patient section
on the lower half of the control and label (+) and tag both patient and
control tissue with Polyvalent (cocktail of IgG,IgM,IgA).
The other control we label (-) and place a pt section on the lower part
as well and leave in pbs and Coverslip.
Does anyone charge for these controls.
Our fee for immunofluorescent tagging is $100.00 each antigen. (I
understand that the AFIP charges $157.00 for each antigen) Any imput
will surely be appreciated.
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