[Histonet] zincTris fixation on frozen sections
Dear All,
I am in the process of staining mouse kidney samples containing grafted
mouse pancreatic islets with antibodies against MHC H2Dd and H2Db.
Tissue samples available are either FFPE or unfixed, snap frozen. I
decided to concentrate on the frozen samples due to the general
difficulty of staining for surface markers after formalin fixation. My
questions are:
1. If I want to use frozen sections, immediately fixed in zincTris
(formalin-free), will I need to process the slides through increasing
alcohols just as suggested for zincTris fixation of tissue samples ?
2. Will zincTris fixation yield better morphology compared to
conventional acetone fixation ?
Yves Heremans
University of Minnesota
Stem Cell Institute
Tel 612-625-0964
Fax 612-624-2436
Address for US Postal Mail:
University of Minnesota
MMC 716
420 Delaware Street SE
Minneapolis, MN 55455
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Suite 14-285 Moos Tower
515 Delaware Street SE
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