Is there a basic protocol for doing fluorescent IHC on frozen or
paraffin sections? Is it better to use a primary antibody conjugated
with FITC or whatever, instead of with the secondary? I never thought it
would be such a headache figuring out fluorescence staining on sectioned
tissues as opposed to cells. Also, do you need to get all the reagent
separate, or can some of the detections kits be used for fluorescence?
Renee' Till, HT
Arkansas Childrens Nutrition Center
Little Rock, AR
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