[Histonet] beta galactosidase help!
i need urgent help with beta galactosidase detection in Bouin's fixed
testes sections (frozen and paraffin)!
Testes got first fixed in Bouin's, then embedded in paraffin and O.C.T,
Staining with X-gal on frozens didn't work? Does someone has any idea if
the fixative (contains formaldehyde, picric acid, glacial acetic acid)
destroys the enzyme?
Does X-gal work on already paraffin embedded sections?
Does someone know a good polyclonal anti beta- galactosidase antibody
which i could use for the paraffin sections?
ANY help is highly appreciated!!!
Histonet mailing list
<< Previous Message | Next Message >>