I am looking for information regarding Methyl Green-Pyronin staining for
nucleic acids.  I have contacted Rowley Biochemical Inc (Danvers, MA) about
their Methyl Green Pyronin stain and Differentiating Solution.  They
provided me with the chemical composition of these solutions and also the
protocol 
for Taft's Method  for Nucleic Acids.  Does anybody routinely use these
items and this method?  Also, have you used them on B5-fixed tissue?  
Any information and/or suggestions would be much appreciated.  Thank you for
your time!

Sunni R. Farley
Histotechnician
Pathology Department 
Seattle Cancer Care Alliance
825 Eastlake Ave East
Seattle, Washington 98109
(206) 288-1312



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