[Histonet] Secondary antibody-HRP conjugates

From:"McNaughton, Euan"

Hi All,

Has anyone had any experience with Zymed SuperPicTure or other kits in
which HRP is directly conjugated to the secondary antibody? I can see
why it'd be useful for tissues that have high levels of endogenous
biotin, but I'm curious about their claims of "superior sensitivity" for
general use.  I'm quite new to the IHC game, but I thought that the
avidin-biotin system was used to improve senstivity, and so removing it
would have the opposite effect...

Any comments would be appreciated!


Euan McNaughton

Attention: The information contained in this message and/or attachments
from AgResearch Limited is intended only for the persons or entities
to which it is addressed and may contain confidential and/or privileged
material. Any review, retransmission, dissemination or other use of, or
taking of any action in reliance upon, this information by persons or
entities other than the intended recipients is prohibited by AgResearch
Limited. If you have received this message in error, please notify the
sender immediately.
Histonet mailing list

<< Previous Message | Next Message >>