[Histonet] Re: Frozen sections fixed or unfixed for IFA

From:Gayle Callis

WE do it both ways with success but HOW you store and handle your sections=20
is important.  I think we get our best fluorescent staining on fresh FS air=20
dried overnight, then fixed the next day followed by immediate IFA 
staining.   The sections MUST be dry before fixation, no matter which way 
you handle them.

At 04:06 AM 4/4/2005, you wrote:
>Hi
>Asking this question for a colleague who does the flourescent staining
>on frozen sections.Does it make a difference to fluorescent staining if
>one fixes cryosections in acetone before freezing the slides at -80C?
>Is it better to rather store the sections unfixed before freezing? Thank
>you
>Marilyn
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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