[Histonet] RE: Staining...

From:"C.M. van der Loos"


   Hi Massimo,

   You're sure your colleagues didn't make a late April 1st joke with
   you?

   In terms of immunohistochemistry the cyan - green color can be
   either peroxidase activity with tetramethylbenzidine as chromogen or
   beta-galatosidase activity with X-gal+ferri-ferro cyanide as
   chromogen.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389


   ----- Original Message -----
      From  "Massimo" 
      Date  Wed, 6 Apr 2005 15:40:56 +0200
        To  "ML Histonet" 
   Subject  [Histonet] Staining...

   Torino 06 April 2005
   (ITALY)
   Greetings to everyone,
   I  am  looking  at  an optical microscope a prepared slice with a thin
   section of a rat kidney.
   I do not know the origin of the specimen.
   Its colour in transmitted light is between cyan and green.
   Does someone tell me what kind of staining has been used?
   Any suggestions would be appreciated.
   Thank you.
   Best Regards,
   Massimo



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