[Histonet] RE: Recycler
We have been using the CBG recycler for about 5 years and have had no
problems with it so far. The recycled product works great and it is a
great cost saver.
Terri Clarke
Histology Supervisor
All Saints Laboratory
3801 Spring Street
Racine, Wi 53405
mclarke@allsaintshealthcare.org
262-687-6609
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, April 12, 2005 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 17, Issue 20
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Today's Topics:
1. Immunohistochemical staining interference by ethanol vs
alcohol blend (Malek, Jack M)
2. formula 83 (Sue Barnes)
3. Ethanol Interference with Immunohistochemical staining
(Malek, Jack M)
4. looking for suggestions on distiguishing protozoa. (HCS)
5. p16 source (Luis Chiriboga)
6. RE: Is TUNEL a waste of time? (Satoshi Akima)
7. Re: Tissue Micro Arrayer (Histo Jock)
8. Embedding Center (Steven Coakley)
9. RE: formula 83 (Christine Tambasco)
10. Re: microtome question (Christine Tambasco)
11. Re: microtome question (Jacqueline Lair)
12. Formula 83 (Kerry Patience)
13. Delaware diamond knives (Burns, Julie)
14. Porcine macrophages (Jens Lindegaard)
15. WT1 (Prada-Puentes Carlos (RW6) PAHNT)
16. Known Gastrin Controls (Histology SLU)
17. vendors (Kapoor, Sue)
18. RE: Porcine macrophages (Patsy Ruegg)
19. Re: vendors (meyenhmf@umdnj.edu)
20. Recycling (Rush, Joyce)
21. Tape transfer sections and IHC (Patsy Ruegg)
22. RE: Recycling (Connie Sims)
23. RE: Porcine macrophages (Favara, Cynthia (NIH/NIAID))
----------------------------------------------------------------------
Message: 1
Date: Mon, 11 Apr 2005 11:09:47 -0700
From: "Malek, Jack M"
Subject: [Histonet] Immunohistochemical staining interference by
ethanol vs alcohol blend
To:
Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202121AC6@ROC2T9.ghc.org>
Content-Type: text/plain; charset="us-ascii"
Has anyone experienced interference with immunohistochemical staining
when using an ethanol blend versus an alcohol blend? We are preparing
to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to
their Dehydrant (ethanol/isopropanol/methanol blend).
Thank you in advance,
Jack Malek
GHC Pathology
Seattle, Washington
206-326-3251
Malek.j@ghc.org
------------------------------
Message: 2
Date: Mon, 11 Apr 2005 14:58:49 -0400
From: "Sue Barnes"
Subject: [Histonet] formula 83
To:
Message-ID:
Content-Type: text/plain; charset="Windows-1252"
Hi,
Has anyone had any experience with the formula 83 replacement for
xylene?
Received a flyer on it and was wondering if anyone was using it yet.
Sue Barnes CT,HT (ASCP)
sbarnes@elch.org
East Liverpool City Hospital
East Liverpool, Ohio
------------------------------
Message: 3
Date: Mon, 11 Apr 2005 12:16:30 -0700
From: "Malek, Jack M"
Subject: [Histonet] Ethanol Interference with Immunohistochemical
staining
To:
Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202B12539@ROC2T9.ghc.org>
Content-Type: text/plain; charset="us-ascii"
Has anyone experienced interference with immunohistochemical staining
when using an ethanol blend versus an alcohol blend? We are preparing
to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to
their Dehydrant (ethanol/isopropanol/methanol blend).
Thank you in advance,
Jack Malek
GHC Pathology
Seattle, Washington
206-326-3251
Malek.j@ghc.org
------------------------------
Message: 4
Date: Mon, 11 Apr 2005 13:12:15 -0700
From: "HCS"
Subject: [Histonet] looking for suggestions on distiguishing protozoa.
To: "Histonet"
Message-ID: <000601c53ed2$c1e23990$6601a8c0@hp>
Content-Type: text/plain; charset="iso-8859-1"
Does someone have a suggestion for a stain that would distinguish
bacteria from protozoa?
Thanks in advance
LeRoy Brown
HCS
www.histocs.com
360-966-7300
------------------------------
Message: 5
Date: Mon, 11 Apr 2005 10:20:26 -0400
From: Luis Chiriboga
Subject: [Histonet] p16 source
To: Histonet
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi everyone
Can anyone recommend a source for anti-p16 for use in human frozen
tissue.......?
TIA
`Luis
------------------------------
Message: 6
Date: Tue, 12 Apr 2005 08:35:27 +1000
From: Satoshi Akima
Subject: [Histonet] RE: Is TUNEL a waste of time?
To: histonet@lists.utsouthwestern.edu
Message-ID: <0787a008ede14e834647a585c31a69e2@bigpond.net.au>
Content-Type: text/plain; charset=US-ASCII; format=flowed
On 11/04/2005, at 7:54 PM, Edwards, R.E. wrote:
> We abandoned the TUNEL method, many years ago as we felt it
> gave too many false +ves, we used H@E staining until reliable
> antibodies came along, e.g., caspase 3............
I have found others who use TUNEL staining express a similar opinion
about the false positives. I would be interested to know if others too
have found an excessive rate of false positives.
Still the TUNEL method seems to be viewed by a great many as some sort
of Gold Standard. Many find still swear by it as a very good tool.
Regarding the issue of specificity - does caspase-3 also help to
distinguish between apoptosis and necrosis I wonder?
Toshi Akima
PhD Student
Centre for Transplantation and Renal Research
Westmead Millenium Institute
Sydney, Australia
Toshi
------------------------------
Message: 7
Date: Mon, 11 Apr 2005 18:41:48 -0400
From: "Histo Jock"
Subject: [Histonet] Re: Tissue Micro Arrayer
To: histonet@lists.utsouthwestern.edu
Cc: Charlene.Henry@STJUDE.ORG
Message-ID:
Content-Type: text/plain; format=flowed
Hi Charlene,
I assume that you ordered a Beecher MTA-II? If so you should expect a
wait.
Beecher's orders for that model are running months ahead of production.
I
understand that it's been a very popular machine. If you ask them nicely
they will send you an MTA-I to use until your MTA-II is ready or you can
just change the order to an MTA-I. For most labs the MTA-I is more than
you
need anyway. Beecher normally has long wait times for everything they
make.
Beecher is the only source for tissue arrayers as they own all of the
patents on them. Chemicon makes an arrayer by licensing a design from
Beecher but as noted already it is both inferior to and hugely more
expensive than the Beecher models ($60K!!! Chemicon vs. $10-20k
Beecher).
Nobody who really knows tissue arrays buys the Chemicon. I've never
heard
anybody speak highly of it.
HistoJock
>Hi Folks,
>Does anyone know of another vendor that makes the tissue micro
>arrayer other than Beecher Instrument. I placed an order with
>Beecher
>Instrument in November, 2004 and they are telling us that it will still
be
>2-3
>months before it will be shipped. At this point I'm willing to look
for
>another vendor.
>Thanks,
>
>Charlene Henry HT (ASCP), QIHC
_________________________________________________________________
Don't just search. Find. Check out the new MSN Search!
http://search.msn.click-url.com/go/onm00200636ave/direct/01/
------------------------------
Message: 8
Date: Mon, 11 Apr 2005 18:02:12 -0500
From: "Steven Coakley"
Subject: [Histonet] Embedding Center
To:
Message-ID: <003901c53eea$8f6bc040$5e81b542@oemcomputer>
Content-Type: text/plain; charset="iso-8859-1"
Our Labs looking for a smaller size used or refurbished embedding
center.
Thanks
------------------------------
Message: 9
Date: Tue, 12 Apr 2005 00:29:34 +0000
From: "Christine Tambasco"
Subject: RE: [Histonet] formula 83
To: SBarnes@elch.org, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format="flowed"
We've been using it now for about a month. We tried it about a
year
ago and finally got approval to switch. I love it! It is almost
as
good as xylene. Good Luck!
Christine Tambasco, HT (ASCP)
ST. Mary's Hospital
427 Guy Park Ave
Amsterdam, New York 12010
>From: "Sue Barnes"
>To:
>Subject: [Histonet] formula 83
>Date: Mon, 11 Apr 2005 14:58:49 -0400
>
>Hi,
>Has anyone had any experience with the formula 83 replacement
for
xylene?
>
>Received a flyer on it and was wondering if anyone was using it yet.
>
>Sue Barnes CT,HT (ASCP)
>
>sbarnes@elch.org
>East Liverpool City Hospital
>East Liverpool, Ohio
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Tue, 12 Apr 2005 00:40:02 +0000
From: "Christine Tambasco"
Subject: Re: [Histonet] microtome question
To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format="flowed"
maybe it's the cassettes that are not fitting right? It's possible
to
get a bad batch. Try it and good luck!
Christine Tambasco, HT (ASCP)
St. Mary's Hospital @ Amsterdam, New York
>From: RCHIOVETTI@aol.com
>To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] microtome question
>Date: Fri, 8 Apr 2005 16:25:21 EDT
>
>In a message dated 4/8/2005 1:07:44 PM US Mountain Standard Time,
>Vickroy.Jim@mhsil.com writes:
>
> > we are having
> > some problems with the block holders not holding the tissue
firm
enough
> > and therefore the blocks can move and therefore chunk. We
have
tried a
> > couple of new holders but are experiencing the same problem.
>
>Jim,
>
>You may already have ruled this out, but I wonder if the
problem
could be in
>the knife holder instead of the cassette clamp? It's strange
that
you get the
>same problem with more than one new specimen holder...nless there
was
some
>kind of manufacturing defect that went undetected, I guess.
>
>My Two Cents' Worth!
>
>Cheers,
>
>Bob
>
>Robert (Bob) Chiovetti, Ph.D.
>Independent Consultant for The Science, Technology and
Industrial
Sectors
>132 North Elster Drive
>Tucson, AZ 85710-3212 USA
>Tel./Fax 520-546-4986
>Member, Arizona Small Business Association - ASBA
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Mon, 11 Apr 2005 18:59:37 -0700 (PDT)
From: Jacqueline Lair
Subject: Re: [Histonet] microtome question
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050412015938.34862.qmail@web81509.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
I was having a similar problem with the microtomes in
our lab.. the block would chunk out the tissue and the
block seemed as if it were barely held into the block
holder, very "wobbly like".
You could wiggle the block around in the block holder
after it was clamped in.
This is what I did:
Look at the block holder as its held or in the "open"
position you would need to have it to insert a block.
You should see one or two small openings (the
"openings" will either be on the bottom or on the
sides of the block holder, depending on which type of
microtome you are using.) between the metal on the
block holder; i.e. where the metal would come or clamp
together to hold a paraffin block.
Next, take a pick and scrape the paraffin out of the
"opening(s)" in the block holder.
When I did the scraping, my block holder had a bunch
of paraffin built up "inside" of it, which was causing
the block holder to not clamp down as tightly or
securely on the block.
I find that if I face in many blocks at one time, I
have to scrape out the block holder after Ive finished
all of my facing.
Now, Ive just gotten into the habit of scraping out
the block holder after Im done facing to ensure that
there is no paraffin build up within the block holder
at all.
I hope this makes sense?
I hope it helps.
Jacqueline Lair, HT (ASCP)
------------------------------
Message: 12
Date: Tue, 12 Apr 2005 17:25:56 +1200
From: "Kerry Patience"
Subject: [Histonet] Formula 83
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Is formula 83 a xylene substitute? What company produces it and
how does the price
compare to xylene?
------------------------------
Message: 13
Date: Tue, 12 Apr 2005 09:29:02 +0100
From: "Burns, Julie"
Subject: [Histonet] Delaware diamond knives
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi
I work in a busy electron microscopy unit and I am considering the
purchase
of a delaware diamond knive - I was wanting to know of anyones
experiences
of these diamond knives ?
many thanks
Julie Burns
------------------------------
Message: 14
Date: Tue, 12 Apr 2005 15:13:35 +0200
From: "Jens Lindegaard"
Subject: [Histonet] Porcine macrophages
To: histonet@lists.utsouthwestern.edu
Message-ID:
Hello
Can anyone tell me which antibody to use in order to immunostain porcine
macrophages in paraffin embedded sections?
Jens Lindegaard M.D.
Eye Pathology Institute
Frederik V's Vej 11, 1.floor
DK-2100 Copenhagen
Denmark
Phone +45 3532 6083
Fax +45 3532 6080
------------------------------
Message: 15
Date: Tue, 12 Apr 2005 14:59:39 +0100
From: "Prada-Puentes Carlos \(RW6\) PAHNT"
Subject: [Histonet] WT1
To: "HISTONET"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi there,
I have been using WT1 with good results from Santa Cruz biotechnology.
My problem is that after 6 months the antibody has become unstable and
altough we have concentrated it the nuclear staining is weaker and
weaker. The datasheet does not recommend freezing and gives ONLY ONE
YEAR!!!! Of guaranteed stability kept at 4 C..Should I freeze it when
the new vial comes? Considering we use at a dilution of 1/3000 we need
to freeze before we use it all.
Thank you to all
Dr Carlos Prada-Puentes
------------------------------
Message: 16
Date: Tue, 12 Apr 2005 07:01:15 -0700 (PDT)
From: Histology SLU
Subject: [Histonet] Known Gastrin Controls
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050412140115.83247.qmail@web51007.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello All:
Does anyone have any extra human gastrin IHC control blocks that you can
spare? Thanks in advance for your help and have a wonderful day!
Susan
---------------------------------
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
------------------------------
Message: 17
Date: Tue, 12 Apr 2005 10:01:14 -0500
From: "Kapoor, Sue"
Subject: [Histonet] vendors
To: histonet@lists.utsouthwestern.edu
Message-ID:
<61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org>
Content-Type: text/plain; charset=iso-8859-1
To all vendors, I'm looking for a used fume hood for a Tissue-Tek
DRS-601
slide stainer and a used Tissue-Tek Cryo Console.
Please email me at:
sue.kapoor@uhsi.org
Thank you,
Sue Kapoor, HT (ASCP)
Histology Coordinator
Kenosha Medical Center
Kenosha, WI
262-653-5570
------------------------------
Message: 18
Date: Tue, 12 Apr 2005 09:46:35 -0600
From: "Patsy Ruegg"
Subject: RE: [Histonet] Porcine macrophages
To: "'Jens Lindegaard'" ,
Message-ID: <200504121546.j3CFkQOD004555@chip.viawest.net>
Content-Type: text/plain; charset="US-ASCII"
I use RDI-Macropabm-387 (Mac387)
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens
Lindegaard
Sent: Tuesday, April 12, 2005 6:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Porcine macrophages
Hello
Can anyone tell me which antibody to use in order to immunostain porcine
macrophages in paraffin embedded sections?
Jens Lindegaard M.D.
Eye Pathology Institute
Frederik V's Vej 11, 1.floor
DK-2100 Copenhagen
Denmark
Phone +45 3532 6083
Fax +45 3532 6080
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 12 Apr 2005 11:49:02 -0400
From: meyenhmf@umdnj.edu
Subject: Re: [Histonet] vendors
To: "Kapoor, Sue"
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <1113320942.425bedeee6f1a@webmail.umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1
I have a slightly used (1 year in a small hospital) ^)! with hood
available.
Also lots of other EM, Histo and Lab Equipment.
Please contact me at: micro@superlink.net
Regards,
Markus F. Meyenhofer
Microscopy Labs
micro@superlink.net
Quoting "Kapoor, Sue" :
> To all vendors, I'm looking for a used fume hood for a Tissue-Tek
> DRS-601
> slide stainer and a used Tissue-Tek Cryo Console.
>
> Please email me at:
> sue.kapoor@uhsi.org
>
> Thank you,
> Sue Kapoor, HT (ASCP)
> Histology Coordinator
> Kenosha Medical Center
> Kenosha, WI
> 262-653-5570
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
------------------------------
Message: 20
Date: Tue, 12 Apr 2005 10:55:04 -0500
From: "Rush, Joyce"
Subject: [Histonet] Recycling
To:
Message-ID:
<2337362E8548BE4C85EE5DD5D526B0851942B4@sjw3smail2.SJMCMN.ORG>
Content-Type: text/plain; charset="iso-8859-1"
We are looking into recycling our alcohol, formalin and Americlear. I'd
love to hear any experiences doing this. We are a relatively small user
and currently sewer everything. I'm trying to gather information for
the day when we can not do this.
Thanks,
Joyce
Joyce A. Rush, BS, MT(ASCP)
Laboratory Manager
St Joseph's Medical Center
523 North Third Street
Brainerd, MN 56401
Office:218-828-7500 Fax: 218-828-7510
------------------------------
Message: 21
Date: Tue, 12 Apr 2005 10:11:10 -0600
From: "Patsy Ruegg"
Subject: [Histonet] Tape transfer sections and IHC
To:
Message-ID: <200504121611.j3CGB1OD014811@chip.viawest.net>
Content-Type: text/plain; charset="US-ASCII"
I am doing hrp IHC on tape transfer sections and I am experiencing a
problem
with unwanted staining on the coating on the polymer coated slides.
Does
anyone else out there do IHC on the tape sections experience this
problem?
Please suggest a solution to this.
Thank you,
Patsy
------------------------------
Message: 22
Date: Tue, 12 Apr 2005 11:18:39 -0500
From: "Connie Sims"
Subject: RE: [Histonet] Recycling
To: histonet@lists.utsouthwestern.edu
Message-ID:
<11906C81B7B03E47A6F10CE5F4784449018A9C1F@dchexch01.driscoll.dch>
Content-Type: text/plain; charset=us-ascii
We currently have a CBG recycler. I love it! It does take some tech time
and planning but the cost savings and environmental benefits are worth
it. Formalin requires rebuffering and alcohol is only 95%. Americlear
is completely reuseable. I haven't had any problems so far, its been
almost a year now. Good luck.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush,
Joyce
Sent: Tuesday, April 12, 2005 10:55 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycling
We are looking into recycling our alcohol, formalin and Americlear. I'd
love to hear any experiences doing this. We are a relatively small user
and currently sewer everything. I'm trying to gather information for
the day when we can not do this.
Thanks,
Joyce
Joyce A. Rush, BS, MT(ASCP)
Laboratory Manager
St Joseph's Medical Center
523 North Third Street
Brainerd, MN 56401
Office:218-828-7500 Fax: 218-828-7510
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 23
Date: Tue, 12 Apr 2005 12:52:37 -0400
From: "Favara, Cynthia (NIH/NIAID)"
Subject: RE: [Histonet] Porcine macrophages
To: 'Patsy Ruegg' , 'Jens Lindegaard'
, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain
Have you looked into F4/80?
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
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-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@ihctech.net]
Sent: Tuesday, April 12, 2005 8:47 AM
To: 'Jens Lindegaard'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Porcine macrophages
I use RDI-Macropabm-387 (Mac387)
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens
Lindegaard
Sent: Tuesday, April 12, 2005 6:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Porcine macrophages
Hello
Can anyone tell me which antibody to use in order to immunostain porcine
macrophages in paraffin embedded sections?
Jens Lindegaard M.D.
Eye Pathology Institute
Frederik V's Vej 11, 1.floor
DK-2100 Copenhagen
Denmark
Phone +45 3532 6083
Fax +45 3532 6080
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 17, Issue 20
****************************************
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