Hi,
     
Good   question!   
   
Actually  we  never  tried  to  fix&n=  bsp;cryo's in
   acetone  before  storage  at  -80C. The only t= hing I do
   know  is  that  proper  storage at -80C of unfixed cryostat section= s
   works  well for a long time. Storing them unfixed gives you at least
   t=  he opportunity  to  choose  the  fixative you like. For
   example,=  we  like  to  fix cryo's  for staining nuclear
   antigens  preferabl= y not in acetone. 2-3 min in NBF works
   much  better  in  this  res= pect.
 
Chris van der Loos, PhD
D= ept. of
   Pathology
Academical   Medical   Center   M2-230
Meiber   gdreef         9
NL-1105         AZ        Amsterdam
The
   Netherlands<=     /P>    
phone:          +31        20        5665631<   BR>fax:               +31            20
   6960389
e-mail:= c.m.vanderloos@amc.uva.nl<   /FONT>
          
 
  
-----         Original         Message         -----
             
             
From    
        
Tyler                          <mtyler@uctgsh1.   uct.ac.za>
    
   
Date    
        
Mon,      04      Apr      2005      12:06:16      +   0200
            
            
To    
        
histonet                          <histonet@lists   .utsouthwestern.edu>
  

   
Subject&nb=             sp;
   
[Histonet]    Frozen
   sections<=        /FONT>
   
Hi
Asking this question
   for=  a  colleague  who does the flourescent staining
on frozen
   sections.=  Does  it  make  a  difference  to  fluorescent  staining
   if
one  fixes  cry=  osections  in  acetone before freezing the
   slides  at  -80°C?
Is  i=  t  better  to  rather  store the
   sections        unfixed       before       freezing?       Thank<   BR>you
Marilyn

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