[Histonet] RE: Frozen sections

From:"C.M. van der Loos"



Good question!

Actually we never tried to fix&n= bsp;cryo's in acetone before storage at -80C. The only t= hing I do know is that proper storage at -80C of unfixed cryostat section= s works well for a long time. Storing them unfixed gives you at least t= he opportunity to choose the fixative you like. For example,= we like to fix cryo's for staining nuclear antigens preferabl= y not in acetone. 2-3 min in NBF works much better in this res= pect.

Chris van der Loos, PhD
D= ept. of Pathology
Academical Medical Center M2-230
Meiber gdreef 9
NL-1105 AZ Amsterdam
The Netherlands
<= /P>

phone:  +31 20 5665631< BR>fax:    +31 20 6960389
c.m.vanderloos@amc.uva.nl< /FONT>


----- Original Message -----

From  Tyler <mtyler@uctgsh1. uct.ac.za>
Date  Mon, 04 Apr 2005 12:06:16 + 0200
To  histonet <histonet@lists .utsouthwestern.edu>
Subject&nb= sp; [Histonet] Frozen sections<= /FONT>

Asking this question for= a colleague who does the flourescent staining
on frozen sections.= Does it make a difference to fluorescent staining if
one fixes cry= osections in acetone before freezing the slides at -80C?
Is i= t better to rather store the sections unfixed before freezing? Thank< BR>you

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