|From:||"C.M. van der Loos" |
Actually we never tried to fix&n= bsp;cryo's in acetone before storage at -80C. The only t= hing I do know is that proper storage at -80C of unfixed cryostat section= s works well for a long time. Storing them unfixed gives you at least t= he opportunity to choose the fixative you like. For example,= we like to fix cryo's for staining nuclear antigens preferabl= y not in acetone. 2-3 min in NBF works much better in this res= pect.
Chris van der Loos, PhD
phone: +31 20 5665631< BR>fax: +31 20
----- Original Message -----
|From||Tyler <mtyler@uctgsh1. uct.ac.za>|
|Date||Mon, 04 Apr 2005 12:06:16 + 0200|
|To||histonet <histonet@lists .utsouthwestern.edu>|
|Subject&nb= sp;||[Histonet] Frozen sections<= /FONT>|
Asking this question for= a colleague who does the flourescent staining
on frozen sections.= Does it make a difference to fluorescent staining if
one fixes cry= osections in acetone before freezing the slides at -80°C?
Is i= t better to rather store the sections unfixed before freezing? Thank< BR>you