For what purpose, antigen retrieval OR decalcification of bone?

Personally, any solutions made up in our go into distilled or equivalent 
water, tap water is reserved for rinsing sections or NBF fixed bone samples.

At 06:52 AM 4/14/2005, you wrote:
>Is it necessary to use distilled H20 for EDTA or can you use tap?
>
>Is anyone using heat with EDTA?  Please send details.
>
>Thank you,
>
>Trisha Emry
>U of WA, Seattle
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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