Re: [Histonet] tissue slicer
Almost forgot ...........
There is a device called a "Stadie-Riggs tissue slicer" that will slice
fresh tissue fairly thin, a few hundred microns. Thomas Scientific sells
them, I don't know if anyone else does. A few hundred dollars in US
currency, I think.
Katy Whalley wrote:
>Thanks to everyone for your advice and suggestions. It seems I might have
>to re-think the thickness of the slices required - in retrospect, I was
>probably aiming too low anyway. Basically, the reason we want to cut them
>quickly is in order to culture the slices later, so I'm not sure a
>vibratome or cryostat would be appropriate as I think the tissue would
>have to be embedded/ frozen first. Overall the 'egg-slicer' or matrix type
>of device seem like they may be the best option, but I may try to make my
>own, as described by Geoff, to cut costs a bit.
> Bad vibes, man.
>>But, Geoff, your device would work, but as you note, not for less
>>than 1000 micron slices, definitely not 30 - 300 micron, and there'd
>>be more tissue damage from compression. A vibratome is pretty much
>>the only choice, there is no "all slices at once" instrument that
>>>Always wondered what a vibratome was.
>>>I've got a wifatome.
>>>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
>>> Consultant Pathologist
>>> Rotherham General Hospital
>>> South Yorkshire
>>>From: Geoff McAuliffe [mailto:firstname.lastname@example.org]
>>>Sent: 19 April 2004 19:08
>>>Subject: Re: [Histonet] tissue slicer
>>> You could buy a Vibratome, a device with a vibrating blade that will
>>>cut fixed or unfixed tissue at a thickness you select. I think there are
>>>several models and vendors.
>>> Or, you could make an inexpensive device for little more than pocket
>>>change. Buy some high-quality double-edge razor blades and some material
>>>to use for spacing the blades. For 1 mm or more use square aluminum rod,
>>>for 0.5 mm or less use "shim stock". A well-stocked hardware store or
>>>maching shop will have these items. Use "super-glue" to glue up a
>>>"blade-spacer-blade-spacer-blade ..." tool with as many blades as your
>>>project demands. One 'application' of the tool to the sample will give
>>>you uniform and reproducable slices. Be sure to cut off or mask the
>>>edge of the blade not in use so you won't cut yourself.
>>>Katy Whalley wrote:
>>>>We are looking for a device which can be used to cut tissue quickly into
>>>>slices of an even thickness. We're not sure yet exactly how thick these
>>>>will be but something in the range 30-300 microns is likely. My
>>>>has in mind something in which several blades are attached to a holder
>>>>that keeps them the correct distance apart, so that all the slices are
>>>>at once. Has anyone ever used/ seen this kind of thing, or anything else
>>>>which would do the job?
>>Supervisor, BBPIC microscopy facility
>>Department of Animal Sciences
>>University of Wisconsin
>>1675 Observatory Drive
>>Madison, WI 53706 - 1284
>>voice: (608) 263-4162
>>fax: (608) 262-5157 (dept. fax)
>>Histonet mailing list
>Histonet mailing list
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
Histonet mailing list
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