Hi Marion,
I think you should do antigen retrieval after deparaffination and than apply
your immunostaining protocol.
Fixation and impregnation with paraffin mask the epitopes and it decreases
the antigenicity.
You can find some information for antigen retrieval.
http://herpesvirus.tripod.com/research/protoc.htm#Antigen%20Retrieval%20for


Good luck!
Petia



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