Re: [Histonet] anti-CD45 antibody for mouse parrafin sections

From:"Petia P Stefanova"

Hi Marion,
I think you should do antigen retrieval after deparaffination and than apply
your immunostaining protocol.
Fixation and impregnation with paraffin mask the epitopes and it decreases
the antigenicity.
You can find some information for antigen retrieval.

Good luck!

Histonet mailing list

<< Previous Message | Next Message >>