Can't you test the residual fixative for the presence or absence of zinc?
My Chemistry is not what it was but can't you do something with an element
and the double decomposition of a salt? Something to do with an element
higher up or lower down the Periodic Table that displaces an element from
a salt that is the other way round, can't remember. But NBF doesn't contain
the heavy metal does it?

Why is it important to know? All I can remember is that zinc formalin made
the tissue brittle, or was that lead? The nuclear stain was rather deep
too. Hope that helps.



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