Re: [Histonet] Testes Bouin's fluid

From:John Kiernan

It does not make sense to put specimens in neutral
buffered formaldehyde after fixing them in Bouin's
fluid. Bouin's is, for many purposes, a better
fixative than NBF because it confers characteristic
patterns of chromatin in nuclei of different cell
types and it also permits brighter staining of
collagen and cytoplasm with anionic dyes. Bouin's
fluid extracts cytoplasmic RNA and it can also
make DNA Schiff-positive (Feulgen hydrolysis), and
can damage red blood cells.

Staining by both haemalum and eosin is stronger
after Bouin's than after NBF, and in many tissues
the microanatomy, as seen in paraffin sections, is 
more lifelike. There is less differential shrinkage
of cells, tubules etc than you see in paraffin
sections of specimens fixed in neutral formaldehyde.  
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
_______________________________ wrote:
> Dear Histonetter,
> We have been preserving testes (of rats and mouse) in bouin's solution and
> after 24 hrs washing them with 70% alcohol and fixing it back in 10%NBF.
> But staining later with H & E is not that great. Can anybody suggest me
> the best protocol for fixing,processing and staining  of testes.
> Dr. Balaji
> Dept. Pre clinical safety evaluation,
> Discovery research,
> Dr. Reddys Laboratories ltd. Bollaram Road,
> Miyapur, Hyderabad, 500 049
>  Andhra Pradseh, INDIA
> _______________________________________________
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