Re: [Histonet] RE: Urea disclosure of endog biotin

From:"Andi Kappeler"

Hi Chris & Carl
Similar results here. I don't worry too much about endogenous biotin after
citrate 6.0, however we fear EDTA 9.0 and even more Tris-Urea 9.5 ...
Sometimes I feel like it should be called immunohistoalchemy rather than
immunohistochemistry ... (never revealed any gold, though)

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
----- Original Message ----- 
From: "C.M. van der Loos" 
Sent: Tuesday, April 20, 2004 1:39 PM
Subject: [Histonet] RE: Urea disclosure of endog biotin

> Hi Carl,
> In my hands on human kidney FFPE sections it's just the other way around:
after citrate 6.0 retrieval there is hardly any endog. biotin (like with
untreated sections) but after EDTA 9.0 there is lots of endog. biotin around
> Who ever said that IHC is a simple and straightforward technique???
> Chris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center
> Amsterdam - The Netherlands
> ----- Original Message ----- 
> >From  Carl 
> Date  Mon, 19 Apr 2004 21:06:04 +0100
> To
> Subject  [Histonet] re Urea disclosure of endog biotin
> Mmmmm...I have to "disclose" that citric acid M/Wing at pH6 does lead to
endog biotin disclosure yet, when I do same with TRIS adjusted to pH10, I
lose practically all endog biotin. (chick tissue being a perfect example)
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