Re: [Histonet] Peeling

From:" Katri Tuomala"

Hi Christian,
Find out what they have in their waterbath, when floating out your sections.
Ask them to use just distilled water with no additives (gelatin ect.). We
used to have tap water with sprinkled gelatin in it and I kept losing my
sections, particularly after HIER.
Since I switched to plain distilled water I hardly ever lose any sections as
long as you drain the water well from underneath the section...
Good luck, I know how frustrating it is.


Katri Tuomala
Department of Pathology
St.Joseph's Health Care
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Christian Franci" 
Sent: Monday, April 19, 2004 5:39 PM
Subject: [Histonet] Peeling

> We've been routinely sending out blocks for sectioning (we're a bit short
> handed at the moment) with out any problems until recently.
> The slides I got back looked fine.  After HIER, though, the tissues on
> just kept peeling off as I was staining them....
> So, I had them sent out again and the sections put on super charged
> Well, while the tissues didn't peel off as much, I still lost a fair
> amount...
> Is there any way to prevent this?
> Any way to reduce peeling after HIER?
> Or, am I *@#!! out of luck?
> My samples represent too much work to watch them being lost.
> Thanks in advance!
> Cheers
> C
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