Re: [Histonet] Oil Red O questions

From:Gayle Callis

Hi Andi, 

I have an oil red O stain that doesn't leave crud all over the slide,
clean, crisp and sooooo easy to do.  I am sending a copy via private email
to you. You will not be unhappy with this stain, it is wonderful and far
less work than you have been doing.  After rinsing, just coverslip with an
aqueous mounting media.  

It was published by Chuck Churukian, and I never looked back once I used
it, NO MORE MESSY stain to deal with! 

Instead of nail polish that contains isopropyl alcohol, dump this out and
put diluted mounting media in little fingernail polish bottle so you can
use tidy little brush.  This seals nicely and dries rapidly. Dilute your
regular mounting media with toluene to consistency of a top coat or base
coat which is thinner than regular nail polish. (Sorry, guys, a girl thing
describing the different types of glamour fingernail polish stuff!)

GFP was found to lose fluorescence due to nail polish that leached into
aqueous media.  Curiousity prompted reading of labeled ingredients on nail
polish bottle.  Except for the alcohol, other ingredients were not miscible
with water, so I deduced the alcohol was the culprit and this was known to
kill glowing GFP, probably not the eGFP of today.  GFP vs nail polish is
published info. 


Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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