Re: [Histonet] BrdU invivo dosage

From:"John C. Dennis"

Dear Dr Balaji

I use 50mg/kg body weight.  I have a reference, not at hand, but will
round it up if you will remind me.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Thu, 15 Apr 2004 wrote:

>    Dear histonetter:
>          Can  anyone  tell me the exact dose of Bromo deoxy uridine in
> rats and mice for the purpose of invivo labelling for immunohistochemistry
> (particularly my interest is intestines  and urinary bladder). We plan to
> carryout both intraperitonially and orally through drinking water.As for
> as I know the dose in mice is 100mg/kg IP at a dose volume of 20 ml/kg. Is
> it OK to  dilute BrdU in only PBS or any other combination is required. .
> It will be of great help for me  if you could provide me the reference
> literature for the same.Thanks in advance.
> Dr. Balaji  (M. V. Sc. Path)
> Dept. Pre clinical safety evaluation,
> Discovery research,
> Dr. Reddys Laboratories ltd. Bollaram Road,
> Miyapur, Hyderabad, 500 049
>  Andhra Pradseh, INDIA
> Tel: 23045439 - Ext.432.
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