Megan,

We're currently using polyclonal laminin from NeoMarkers (Lab Vision) at 1:50 with protease pretreatment and stained on Ventana instruments.  We were using it clinically for basement membrane staining but it seems to be inconsistent in its staining so now we use it as a research tool.  Our pathologists don't feel its a problem with the antibody itself but rather the nature of laminin.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: Megan Kear [mailto:Megan.Kear@hunter.health.nsw.gov.au]
Sent: Wednesday, April 28, 2004 11:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] laminin


Hi Histonetters

Anybody using a good laminin polyclonal antibody. To be used on
formalin fixed paraffin fixed embedded sections.

thank you for your help.

Megan Clarke
HAPS
IHC dept
Newcastle
Australia

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