RE: [Histonet] Re: Zinc formalin and 10% NBF

From:"Johnson, Teri"

I am using Richard Allan's commercial formulation.  They'd tell you what
concentration of Zinc they use, but then they'd have to kill you.  This
could be fodder for the next Mission: Impossible movie. Think Tom Cruise
would go for it?

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
Sent: Wednesday, April 28, 2004 10:09 AM
To: Johnson, Teri;
Subject: RE: [Histonet] Re: Zinc formalin and 10% NBF

"It was amazing to see how firm and grayish the tissue was as compared
to the NBF fixed tissue, within even an hour of fixation time."

I too am eager to learn what formulation you are using. The first time I
tried zinc, (in 1971), I used about 8% (because I had no idea what
strength to use - just trying a poor man's mercury), and the tissue went
the colour and consistency of concrete within half an hour. In fact, it
only takes homeopathic doses of zinc to do the trick.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England

-----Original Message-----
From: Johnson, Teri []
Sent: 27 April 2004 19:15
Subject: [Histonet] Re: Zinc formalin and 10% NBF

In working through our processing schedule dilemma, we fixed some
pancreas in NBF (overnight), and fixed some in Zinc Formalin for 3
hours, followed by water wash and storage in 70% ethanol.  We found that
using a variety of processing schedules, the NBF fixed pancreas looked
good with routine 30 min/station (with 2 hours total time in paraffin),
all the way down to 10 min/station (with 80 min total time in paraffin).
The Zinc Formalin fixed pancreas looks dry and brittle still, even with
the short processing schedule.
We will try a hand-processing schedule for these to see if we can get
the processing perfect for the ZnF pancreas.  Our answer to this problem
could be that we need to limit the amount of time it is in fixative.
Gayle, how long were the kidney biopsies fixed before processing?

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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