RE: [Histonet] Microwave Processing

From:"Steven E. Slap"

Hi Terry & HistoNetters

Yes, in the Leong method for biopsies, the specimens are heat 
stabilized in the microwave in saline, and not really chemically 
fixed.  They get fixed in the ethanol in a traditional processor 
(Leong used ethanol, chloroform and paraffin-  no xylene).  I haven't 
seen the resulting morphology myself.

As for breasts, the Milestone unit has a module, the DDG, which is 
280mm x 250mm, for stabilization of breasts and other large organs. 
One advantage with breasts is that the nodes just light up bright 
white.

Parenthetically, I don't believe that the breasts in Yorkshire could 
possibly be bigger than  in the American South...

best regards,
Steven

>Thanks Steven. It was the Leong method to which I referred.
>I am totally befuddled over fixation in the microwave. Surely in the ...
>...snip...
>... heat fixation?
>
>Firming up breasts in the microwave seems fun - but the breasts in 
>Yorkshire are bigger than the microwaves:-)


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