RE: [Histonet] Microwave Processing
|From:||"Marshall Terry Dr, Consultant Histopathologist" |
Thanks Steven. It was the Leong method to which I referred.
I am totally befuddled over fixation in the microwave. Surely in the Leong method (for blocks), the saline is irrelevent other than as a carrier and buffer medium, and it is heat fixation?
Firming up breasts in the microwave seems fun - but the breasts in Yorkshire are bigger than the microwaves:-)
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Rotherham General Hospital
From: Steven E. Slap [mailto:firstname.lastname@example.org]
Sent: 17 April 2004 22:54
To: Marshall Terry Dr, Consultant Histopathologist; kevin williams;
Subject: RE: [Histonet] Microwave Processing
Hi Terry & HistoNetters
I have done lots of successful large organ
stabilization in saline at 65°C for 20-35 minutes
(depending on the size of the organ. However, it
is important to emphasize that the purpose of
this step usually is to firm up the tissue so
that it can be subsequently cut up into thinner
pieces for processing, which must include a
fixation step, either in the microwave or on a
conventional processor, in formalin or some
non-formalin fixative. The saline will not work
alone as a fixation step, except in the case of
very small biopsies (as written up in an article
by Tony Leong)- a procedure I have not tried
At 3:58 PM +0100 4/16/04, Marshall Terry Dr,
Consultant Histopathologist wrote:
>Sorry to but in - but on a related question -
>does anyone do microwave fixation in saline as
>described by for instance the Melbourne group,
>which involves bringing up to 65C in saline?
>Have tried it this week and the sections are even crummier than usual.
>When I was in Tasmania, a local private lab. did
>it and their sections were fine.
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