RE: [Histonet] Microwave Processing

From:"Willis, Donna"

Terry,
Saline will not fix your tissue.  It will only stabilize it.  If you do not
post fix in formalin after stabilizing in saline then your tissue will be
fixed in the alcohol when processed.  Saline works great to firm up the
tissue to assist in dissection.

Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall
Terry Dr, Consultant Histopathologist
Sent: Friday, April 16, 2004 9:59 AM
To: kevin williams; histonet@pathology.swmed.edu
Subject: RE: [Histonet] Microwave Processing 


Sorry to but in - but on a related question - does anyone do microwave
fixation in saline as described by for instance the Melbourne group, which
involves bringing up to 65C in saline?
Have tried it this week and the sections are even crummier than usual.
When I was in Tasmania, a local private lab. did it and their sections were
fine.

Dr Terry L (why can't I get a good H&E) Marshall, B.A.(Law),
M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: kevin williams [mailto:akwilliams75@hotmail.com]
Sent: 16 April 2004 15:33
To: histonet@pathology.swmed.edu
Subject: [Histonet] Microwave Processing and Derm Shaves



   Is anyone out there doing any processing on derm shaves with microwave
   processing? How is it and how are the results?
   Thanks.

   A. Kevin Williams
   Vermont Dermatopathology.
     _________________________________________________________________

   [1]Get rid of annoying pop-up ads with the new MSN Toolbar FREE!

References

   1. http://g.msn.com/8HMBENUS/2752??PS=
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>