RE: [Histonet] Embedding in OCT and subsequent steps
Actually, that's not completely true. Surgipath makes a mountant/clearing
agent in one called Clearium. I use it instead of xylene or xylene
substitute. Although, I guess in the strictest sense of the word, it is a
"substitute".
-----Original Message-----
From: Charles Scouten [mailto:cwscouten@myneurolab.com]
Sent: Friday, April 30, 2004 2:52 PM
To: degaboh@rice.edu; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Embedding in OCT and subsequent steps
Xylene infiltrates tissue, and prepares it for coverslipping (ie, with
Permount). You still need xylene or a substitute if you plan to coverslip
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
cwscouten@myneurolab.com
http://www.myneurolab.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ZP
Sent: Friday, April 30, 2004 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding in OCT and subsequent steps
If I embed my sample in OCT, do I need to use xylene in later steps (i.e.
when staining or before mounting)? I have a protocol that does that but I
thought xylene was really more for paraffin embedding. Thanks!!
Zarana Patel
degaboh@rice.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>