RE: [Histonet] Embedding in OCT and subsequent steps

From:"Charles Scouten"

 Xylene infiltrates tissue, and prepares it for coverslipping (ie, with Permount).  You still need xylene or a substitute if you plan to coverslip

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 

-----Original Message-----
From: [] On Behalf Of ZP
Sent: Friday, April 30, 2004 12:08 PM
Subject: [Histonet] Embedding in OCT and subsequent steps

If I embed my sample in OCT, do I need to use xylene in later steps (i.e.
when staining or before mounting)?  I have a protocol that does that but I thought xylene was really more for paraffin embedding.  Thanks!!

Zarana Patel

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