Xylene infiltrates tissue, and prepares it for coverslipping (ie, with Permount).  You still need xylene or a substitute if you plan to coverslip


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten@myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ZP
Sent: Friday, April 30, 2004 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding in OCT and subsequent steps

If I embed my sample in OCT, do I need to use xylene in later steps (i.e.
when staining or before mounting)?  I have a protocol that does that but I thought xylene was really more for paraffin embedding.  Thanks!!

Zarana Patel
degaboh@rice.edu


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