I don't use the ARK kit at all.  A wide variety of animal tissues pass
through my lab and I have found either the DAKO LSAB2 (HRP + AEC)or
their Envision polymer (HRP-AEC or DAB) works great. I've never tried
the LSAB2 or Envision AP kits.  I've been tempted to try them. I also
like the serum-free blocker for the rabbit polyclonals I'm working with.
It kills the background staining fabulously.
 
>> Hey, research is fun, and who sez we have to do it one way only! 
 
Gayle --- HA! Isn't life great??  I think I'll go have some fun myself
... |:)
 
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805
 
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Wednesday, April 14, 2004 12:04 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] AEC+ with ARK kit
 
Just curious, has anyone used AEC+ with the DAKO ARK kit during
staining.
Have a researcher who really does NOT like DAB final color, period! We
are
playing with Chris van der Loos's AEC recipe (quite sensitive! very
similar
to AEC+) and although this means tweaking antibody dilution, etc -  all
those good things needed for ARK'ing it seems to be working with our
antibody.  
 
 
 
 
Gayle Callus
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
 
 
 
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